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Description: Provides reconditioned biotechnology and pharmaceutical equipment.
Summary: Home - Labcentraal - Earth's Source for Chromatography, Real Time PCR & Used Lab Equipment
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Information: The FACS Vantage uses electrostatic droplet sorting by the generation of droplets from the core stream. This produces a stable line of droplets at 10,000 to 60,000 Hz, depending upon nozzle size. Larger cells use a larger nozzle and lower drop-drive frequencies and so are sorted at lower optimum rates. To prevent distortion of the laminar flow of the sample/sheath fluid and disruption of the stable droplet break-off (clogging of the Nozzle), it is required that ALL samples be filtered prior to sorting. Sterilized nylon mesh (20 - 70 uM pore size) can be used. The number of cells that can be sorted depends upon factors such as frequency of events to be sorted, total number, and required purity. These factors can be discussed prior to sorting. Sheath fluid used is sterile 0.9% Sodium Chloride or PBS. Sterilization of the cell sorter is performed as required using 10 % bleach, 70 % ethanol and distilled water.